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Polymerases

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2X RbTaq™ DyeMIX-Tenuto HOT new

No Product Name Cat.# Conc Size Price(₩) Add
1 2X RbTaq™ DyeMIX-Tenuto HOT P510RB 2X 1 ml ₩ 75,000 추가
2 2X RbTaq™ DyeMIX-Tenuto HOT P525RB 2X 2.5 ml ₩ 150,000 추가
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Product description

2X RbTaq™ DyeMIX-Tenuto HOT is similarly formulated to the 2X RbTaq™ PreMIX-Tenuto HOT except that it contains loading dyes for further convenience of use. Thus, the reaction mixtures after PCR cycles are ready for agarose gel electrophoresis. This product includes RbTaq™ Tenuto HOT DNA Polymerase (Cat. P225RB), and is optimized to amplify DNA longer than 10 kb, which is difficult with common Taq polymearases. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products.

 

Characteristics

- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)

- A-tail formation at 3’ ends of amplified duplex DNA

 

Applications

- Amplification of long DNA fragments (<10 kb)

- Amplification of high-complexity template DNA

- Primer extension

- Colony PCR

 

Components(2X)

- RbTaq™ Tenuto HOT DNA Polymerase: 0.2 unit/μl

- RbTaq™ Tenuto HOT buffer (containing 4 mM Mg2+)

- dNTP mixture: 0.4 mM each

- Stabilizer

- Dyes: Xylene cyanol and Orange G

 

Storage

Stable up to 18 months at -20 or 3 months at 4 (Storage at -20 is recommended).

 

Standard reaction conditions

- PCR mixture

2X RbTaq DyeMIX-Tenuto HOT

10 μl

Template DNAa (0.1~500 ng/μl)

1 μl

Primer 1 (5 pmole/μl)

1 μl

Primer 2 (5 pmole/μl)

1 μl

Sterile water

up to 20 μl

aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

 

- PCR cycles

Step

Temperature

Time

Cycle

Initial denaturationa

95

3 min

1

Denaturation

95

30 sec

25~35

Annealingb

55~65

30~60 sec

Elongation

72

1 min/kb

Final elongation

72

5 min

1

When cycles are over, keep the reaction at 4; may add 10 mM EDTA until use to prevent DNA degradation

a30 sec at 95 for cDNA and 3 min at 95 for genomic DNA is recommended for enzyme activation.

bRecommend annealing temperature is 5 to 10 below the lower Tm of the two primers used.

 

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