Product description

2X RbTaq™ DyeMIX-Tenuto HOT is similarly formulated to the 2X RbTaq™ PreMIX-Tenuto HOT except that it contains loading dyes for further convenience of use. Thus, the reaction mixtures after PCR cycles are ready for agarose gel electrophoresis. This product includes RbTaq™ Tenuto HOT DNA Polymerase (Cat. P225RB), and is optimized to amplify DNA longer than 10 kb, which is difficult with common Taq polymearases. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products.

Characteristics

- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)

- A-tail formation at 3’ ends of amplified duplex DNA

Applications

- Amplification of long DNA fragments (<10 kb)

- Amplification of high-complexity template DNA

- Primer extension

- Colony PCR

Components(2X)

- RbTaq™ Tenuto HOT DNA Polymerase: 0.2 unit/μl

- RbTaq™ Tenuto HOT buffer (containing 4 mM Mg²⁺)

- dNTP mixture: 0.4 mM each

- Stabilizer

- Dyes: Xylene cyanol and Orange G

Storage

Stable up to 18 months at -20℃ or 3 months at 4℃ (Storage at -20℃ is recommended).

Standard reaction conditions

- PCR mixture

^aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

- PCR cycles

When cycles are over, keep the reaction at 4℃; may add 10 mM EDTA until use to prevent DNA degradation

^a30 sec at 95℃ for cDNA and 3 min at 95℃ for genomic DNA is recommended for enzyme activation.

^bRecommend annealing temperature is 5 to 10℃ below the lower Tm of the two primers used.

●  Enzynomics_P510RB_2X RbTaq™ DyeMIX–Tenuto HOT_manual

● Material Safety Data Sheet

- Enzynomics_MSDS_P510RB_2X RbTaq™ DyeMIX-Tenuto HOT