-
[18/04/19]
-
[진행] Premade Buffer 출시이벤트 (2018년 6월까지)
[18/04/02]
-
[진행] 2018년 신제품 출시 이벤트_제한효소, Modi...
[18/04/02]
-
[진행] SuperiorScript III Master Mix 출시 이벤...
[18/03/27]
-
[종료] Enzynomics Premade Buffer 출시 이벤트
[18/01/02]
2X RbTaq™ DyeMIX-Tenuto HOT
No | Product Name | Cat.# | Conc | Size | Price(₩) | Add |
---|---|---|---|---|---|---|
1 | 2X RbTaq™ DyeMIX-Tenuto HOT | P510RB | 2X | 1 ml | ₩ 75,000 |
![]() |
2 | 2X RbTaq™ DyeMIX-Tenuto HOT | P525RB | 2X | 2.5 ml | ₩ 150,000 |
![]() |
Product description
2X RbTaq™ DyeMIX-Tenuto
HOT is similarly formulated to the 2X RbTaq™ PreMIX-Tenuto HOT except that it
contains loading dyes for further convenience of use. Thus, the reaction
mixtures after PCR cycles are ready for agarose gel electrophoresis. This
product includes RbTaq™ Tenuto HOT DNA
Polymerase (Cat. P225RB), and is optimized to amplify DNA longer than 10 kb,
which is difficult with common Taq polymearases. Thus, this product is improved
in both fidelity (> 2 fold) of PCR products and amplification efficiency of
longer PCR products.
Characteristics
- 2-Dye system: Easy
for gel electrophoresis (xylene cyanol and Orange G)
- A-tail formation at
3’ ends of amplified duplex DNA
Applications
- Amplification of long
DNA fragments (<10 kb)
- Amplification of
high-complexity template DNA
- Primer extension
- Colony PCR
Components(2X)
- RbTaq™ Tenuto HOT DNA Polymerase: 0.2 unit/μl
- RbTaq™ Tenuto HOT buffer (containing 4 mM Mg2+)
- dNTP mixture: 0.4 mM
each
- Stabilizer
- Dyes: Xylene cyanol
and Orange G
Storage
Stable up to 18 months
at -20℃ or 3 months at 4℃ (Storage at -20℃ is
recommended).
Standard reaction conditions
- PCR mixture
2X RbTaq™
DyeMIX-Tenuto HOT |
10 μl |
Template DNAa (0.1~500 ng/μl) |
1 μl |
Primer 1 (5 pmole/μl) |
1 μl |
Primer 2 (5 pmole/μl) |
1 μl |
Sterile water |
up to 20 μl |
aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng
- PCR cycles
Step |
Temperature |
Time |
Cycle |
Initial denaturationa |
95℃ |
3 min |
1 |
Denaturation |
95℃ |
30 sec |
25~35 |
Annealingb |
55℃~65℃ |
30~60 sec |
|
Elongation |
72℃ |
1 min/kb |
|
Final elongation |
72℃ |
5 min |
1 |
When cycles are over, keep the reaction at 4℃; may add
10 mM EDTA until use to prevent DNA degradation
a30 sec at 95℃ for cDNA and 3 min at 95℃ for
genomic DNA is recommended for enzyme activation.
bRecommend annealing
temperature is 5 to 10℃ below the lower Tm of the two primers used.
● Material Safety Data Sheet