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Polymerases

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T4 DNA Polymerase

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 DNA Polymerase DP004S 3 units/μl 150 units ₩ 68,000 추가
2 T4 DNA Polymerase DP004L 3 units/μl 750 units ₩ 272,000 추가
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Description

T4 DNA Polymerase has 5´→ 3´ polymerase activity and 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). However, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

 

Characteristics

- Extreme fidelity

- Gap filling (no strand displacement activity)

- Best enzyme for creating blunt ends

- Isolated from a recombinant source

 

Activity

- 5’→3’ exonuclease: No

- 3’→5’ exonuclease: Yes

- Strand Displacement: No

 

Applications

- 3´ overhang removal to form blunt ends

- 5´ overhang fill-in to form blunt ends

- Single strand deletion subcloning

- Second strand synthesis in site-directed mutagenesis

- Probe labeling using replacement synthesis

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C

 

Reaction Conditions

1X T4 DNA polymerase buffer, 1X BSA, Incubate at 12

 

Storage Conditions

100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C, Store at -20°C

 

Heat Inactivation

75°C for 20 min

 

Quality Control

- Endonuclease-free

 

Supplied with

- 10X T4 DNA Polymerase Buffer

- 100X BSA (10 mg/ml)

- Sterile water

 

Cautions

- dNTP concentrations may differ for different protocols.

- T4 DNA Polymerase is active in all four EzBuffers as well as T4 DNA Ligase Reaction Buffer with dNTPs 

  and BSA.

- Heat inactivate T4 DNA Polymerase at 75°C for 20 minutes with 10 mM EDTA.


 

● Material Safety Data Sheet

- Enzynomics_MSDS_DP004_T4 DNA Polymerase

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