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Polymerases

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Klenow DNA Polymerase
(DNA polymerase l large fragment)

No Product Name Cat.# Conc Size Price(₩) Add
1 Klenow DNA Polymerase
(DNA polymerase l large fragment)
KP001S 5 units/ μl 200 units ₩ 80,000 추가
2 Klenow DNA Polymerase
(DNA polymerase l large fragment)
KP001M 5 units/ μl 400 units ₩ 144,000 추가
3 Klenow DNA Polymerase
(DNA polymerase l large fragment)
KP001L 5 units/ μl 1,000 units ₩ 320,000 추가
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Product description

Klenow DNA Polymerase is a truncated version of E. coli DNA polymerase I, which lacks the 5'->3' exonuclease activity. Since it contains intact 3'->5' proofreading exonuclease activity in addition to

5'->3' polymerase, its fidelity of DNA synthesis equals to that of the full-length E. coli DNA polymerase I. It also contains strand displacement activity for nick translation.

 

Characteristics

- Molecular weight: 68 KDa

- Reaction temperature: 25

- Heat inactivation: 75, 20 min

- Specific activity: 20,000 units/mg

 

Activity

- 5’→3’ exonuclease: No

- 3’→5’ exonuclease: Yes

- Strand Displacement: Yes

 

Applications

- Radioisotope labeling of double stranded DNA with recessed 3' ends.

- Filling-in of 5' overhangs to produce blunted duplex ends

- Removal of 3' overhangs to produce blunted duplex ends

- Second strand synthesis of cDNA obtained by reverse transcriptase

- Radioisotope labeling of DNA with random primers

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

 

Unit definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble materials with 70 mg/ml of denatured herring sperm DNA as template in 30 min at 37.

 

Storage buffer

100 mM KPO4 (pH 6.5), 1 mM DTT, 50% glycerol

 

10X Klenow DNA Polymerase buffer

100 mM Tris-HCl (pH 7.9), 100 mM MgCl2, 10 mM DTT, 500 mM NaCl

 

Supplied with

- 10X Klenow DNA Polymerase buffer

- Sterile water

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