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Polymerases

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Bst DNA polymerase (Large fragment)

No Product Name Cat.# Conc Size Price(₩) Add
1 Bst DNA polymerase (Large fragment) DP006S 8 units/ μl 1,600 units ₩ 68,000 추가
2 Bst DNA polymerase (Large fragment) DP006L 8 units/ μl 8,000 units ₩ 272,000 추가
3 Bst DNA polymerase (Large fragment) DP006H 100 units/ μl 8,000 units ₩ 272,000 추가
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Product description

Large Fragment of the Bst DNA Polymerase from Bacillus stearothermophilus is isolated as a recombinant. It lacks the 5´ → 3´ exonuclease domain. Therefore, Large Fragment of the Bst DNA Polymerase catalyzes 5' → 3' synthesis of DNA and but lacks 5' → 3' and 3' → 5' exonuclease activities.

 

Characteristic

- Isolated from a recombinant source

- Sequencing through problematic secondary structures

- Thermophilic DNA polymerase with strong strand displacement activity

 

Activity

- 5’→3’ exonuclease: No

- 3’→5’ exonuclease: No

- Strand Displacement: Yes

 

Applications

- Random-primed DNA labeling

- Labeling by fill-in 5'-overhangs of dsDNA

- Loop-mediated isothermal amplification (LAMP).

- Whole genome amplification (WGA).

- Ramification amplification (RAM).

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

 

Unit definition 

One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Storage buffer

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.4 @ 25°C

 

1X Reaction buffer

20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton® X-100, pH 8.8 @ 25°C

 

Supplied with

- 10X Bst DNA Polymerase(Large) Buffer

- Sterile water

 

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