컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

Polymerases

Home > PRODUCTS > Polymerases

nTaq-Tenuto (UDG plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 nTaq-Tenuto (UDG plus) P225AU 5 units/μl 250 units ₩ 94,000 추가
2 nTaq-Tenuto (UDG plus) P250AU 5 units/μl 500 units ₩ 170,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

nTaq-Tenuto (UDG plus) is nTaq (Cat. P025) supplemented with 3'→5’ proofreading activity and a PCR enhancing factor for improved efficiency and fidelity. nTaq-Tenuto (UDG plus) can be used to amplify DNA longer than 10 kb, which is difficult with common Taq polymerases alone. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products. Also, UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions.

 

Characteristic

- Carry-over contamination control: contains UDG

- Molecular weight: 94 kDa

- Error rate: 3.0 X 10-6

- Thermal stability: Half-life of 40 min at 95

- A-tail formation at 3’ ends of amplified DNA products.

 

Applications

- Amplification of long DNA fragments (<10 kb)

- Amplification of high-complexity template DNA

- Primer extension

- Diagnostic PCR

- Colony PCR

- Multiplex PCR

- Labeling of DNA fragments with radioactive-isotopes

- Nucleotide sequencing

 

Supplied with

- 10X nTaq-Tenuto Buffer (Mg2+ plus)

- dNTP Mixture with dUTP (2 mM each)

- Sterile water

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase free

- Inhibitor-free

 

Unit definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30min at 74 in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl2, 10 mM β-mercaptoethanol, 12.5 μg of calf thymus DNA).

 

Storage buffer

20 mM Tris-HCl (pH 7.9), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween-20, 50% glycerol.

 

Standard reaction conditions

- PCR mixturea

10X nTaq-Tenuto Buffer (Mg2+ plus)

μl

nTaq-Tenuto (UDG plus) DNA polymeraseb (5 units/μl)

0.2 μl

dNTP mixture with UTP (2 mM each)

μl

Template DNA(0.1~500 ng/μl)

μl

Primer 1 (5 pmole/μl)

μl

Primer 2 (5 pmole/μl)

μl

Sterile water

up to 20 μl

aAssemble the reaction mixture on ice

bAdd the PCR polymerase at the final step

cPlasmid DNA, 0.1 ng~30 ng; genomic DNA, 50 ng~500 ng

 

- PCR cycle

Step

Temperature

Time

Cycle

Pre-incubation (for UDG)

25

10 min

1

Initial denaturation

95

2 min

1

Denaturation

95

30 sec

25~35

Annealinga

55~65

30~60 sec

Elongation

72

1 min/kb

Final elongation

72

5 min

1

When cycles are over, keep the reaction mixture at 4; may add 10 mM EDTA until use to prevent DNA degradation.

aRecommended annealing temperatures is 5 to 10 below the lower Tm of the two primers used.

 


● Material Safety Data Sheet

- Enzynomics_MSDS_P225AU_nTaq-Tenuto (UDG plus)

List