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Polymerases

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nTaq-Tenuto (Dye plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 nTaq-Tenuto(Mg2+ plus/Dye plus) P225AD 5 units/μl 250 units ₩ 90,000 추가
2 nTaq-Tenuto (Mg2+ plus/Dye plus) P250AD 5 units/μl 500 units ₩ 162,000 추가
3 nTaq-Tenuto (Mg2+ free/Dye plus) P225BD 5 units/μl 250 units ₩ 90,000 추가
4 nTaq-Tenuto (Mg2+ free/Dye plus) P250BD 5 units/μl 500 units ₩ 162,000 추가
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Product description

nTaq-Tenuto is nTaq (Cat. P025, P050) supplemented with 3'à 5’ proofreading activity and a PCR enhancing factor for improved efficiency and fidelity. nTaq-Tenuto can be used to amplify DNA longer than 10 kb, which is difficult with common Taq polymerases alone. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products.


Characteristics

- Molecular weight: 94 kDa

- Error rate: 3.0 X 10-6

- Thermal stability: Half life of 40 min at 95

- A-tail formation at 3’ ends of amplified DNA products.


Applications

- Amplification of long DNA fragments (<10 kb)

- Amplification of high-complexity template DNA

- Primer extension

- Colony PCR

- Labeling of DNA fragments with radioactive-isotopes

- Nucleotide sequencing

 

Supplied with

nTaq-Tenuto (Mg2+ plus/Dye plus)

- 10X nTaq-Tenuto buffer (Mg2+ plus/Dye plus)

- dNTP Mixture (2 mM each)

- GC Melt I

- GC Melt II

- Sterile water

 

nTaq-Tenuto (Mg2+ free/Dye plus)

- 10X nTaq-Tenuto buffer (Mg2+ free/Dye plus)

- dNTP Mixture (2 mM each)

- GC Melt I

- GC Melt II

- 25 mM MgCl2

- Sterile water


Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase free

- Inhibitor-free


Unit definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30 min at 74 in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl2, 10 mM -mercaptoethanol, 12.5 μg of calf thymus DNA).


Storage buffer

20 mM Tris-HCl (pH 7.9), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween-20, 50% glycerol.


10X nTaq-Tenuto buffer (Dye plus)

Mg2+ plus buffer: Containing 20 mM Mg2+, 10X loading dye

Mg2+ free buffer: 10X loading dye

 

GC Melt l and ll

- This product is useful for amplification of DNA with GCrich sequences or to avoid amplification of

non-specific bands (Use of GC Melt may reduce PCR efficiency).

- In general, use 1X strength in PCR reaction by diluting the 10X solution, but the amount should be

adjusted for optimal results.


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