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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

Apo I new

No Product Name Cat.# Conc Size Price(₩) Add
1 Apo I R121S 10 units/μl 1,000 units ₩ 67,000 추가
2 Apo I R121M 10 units/μl 2,000 units ₩ 121,000 추가
3 Apo I R121L 10 units/μl 5,000 units ₩ 268,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Recognition & cleavage sequence

 

Source

Arthrobacter protophormiae

 

Reaction conditions

- 1X EzBuffer III 50

- 1X FastCut Buffer, 50

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer III

50 mM Tris-HCl (pH 7.9 @ 25), 100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.

 

Storage

10 mM Tris-HCl (pH 7.4), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

 

Dilution buffer

EzDiluent A

 

Heat Inactivation

80°C for 20 min

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 10%

EzBuffer II: 75%

EzBuffer III: 100%

EzBuffer IV: 75%

FastCut Buffer: 100%

 

Note

Cleaves to leave 5´ AATT extension which can be ligated to DNA fragments generated by EcoR I digestion.

 


 


Figure 1. Lambda DNA digested with Apo I


● Material Safety Data Sheet

- Enzynomics_MSDS_R121_Apo I


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