컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

CviA I new

No Product Name Cat.# Conc Size Price(₩) Add
1 CviA I R120S 5 units/μl 200 units ₩ 62,000 추가
2 CviA I R120M 5 units/μl 400 units ₩ 112,000 추가
3 CviA I R120L 5 units/μl 1,000 units ₩ 248,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Recognition & cleavage sequence

 

Source

CviAI gene from Chlorella virus PBCV-1

 

Reaction conditions

- 1X EzBuffer , 37

- 1X FastCut Buffer, 37

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer IV

20 mM Tris-acetate (pH 7.9 @ 25), 50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

 

Storage

10 mM Tris-HCl (pH 7.5), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml BSA, 50% glycerol

 

Dilution buffer

EzDiluent A

 

Heat Inactivation

65°C for 20 min.

 

Methylation sensitivity

dam methylation: Sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 10%

EzBuffer II: 50%

EzBuffer III: 10%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

- It is an isoschizomer of Mbo I.

- DNA cleavage is blocked by dam methylation.



 


Figure 1. Lambda DNA digested with CviA I


●  Enzynomics_R120_CviA I_manual


● Material Safety Data Sheet

- Enzynomics_MSDS_R120_CviA I


List