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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

TspM I new

No Product Name Cat.# Conc Size Price(₩) Add
1 TspM I R119S 5 units/μl 200 units ₩ 80,000 추가
2 TspM I R119M 5 units/μl 400 units ₩ 144,000 추가
3 TspM I R119L 5 units/μl 1,000 units ₩ 320,000 추가
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Recognition & cleavage sequence

Source

Thermus species

 

Reaction conditions

1X EzBuffer , 75

1X FastCut Buffer, 75

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer IV

20 mM Tris-acetate (pH 7.9 @ 25), 50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

 

Storage

20 mM Tris-HCl (pH 8.0), 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol, 0.1% Triton X-100

 

Dilution buffer: EzDiluent B

10 mM Tris-HCl (pH 7.4 @ 25), 300 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

No.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Sensitive

 

Relative activity in EzBuffers

EzBuffer I: 50%

EzBuffer II: 75%

EzBuffer III: 50%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

- It is an isoschizomer of Xma I.

- Incubation, 37°C results in 20% activity.

- May exhibit star activity in EzBuffer , , .




Figure 1. Extreme pure(EP) assay

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 50 U of enzyme for 4 hr.


● Material Safety Data Sheet

- Enzynomics_MSDS_R119_TspM I

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