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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

Mun I new

No Product Name Cat.# Conc Size Price(₩) Add
1 Mun I R118S 10 units/μl 300 units ₩ 45,000 추가
2 Mun I R118M 10 units/μl 600 units ₩ 81,000 추가
3 Mun I R118L 10 units/μl 1,500 units ₩ 180,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Recognition & cleavage sequence

Source

Mycoplasma unidentified

 

Reaction conditions

- 1X EzBuffer , 37

- 1X FastCut Buffer, 37

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer II

10 mM Tris-HCl (pH 7.9 @ 25), 50 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

 

Storage

10 mM Tris-HCl (pH 7.4), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

 

Dilution buffer

EzDiluent A

 

Heat Inactivation

Mun I can be inactivated at 65 for 20 min.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 100%

EzBuffer II: 100%

EzBuffer III: 10%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

It is an isoschizomer of Mfe I.

 

● Material Safety Data Sheet

- Enzynomics_MSDS_R118_Mun I

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