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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

Lsp1109 I

No Product Name Cat.# Conc Size Price(₩) Add
1 Lsp1109 I R113S 5 units/μl 200 units ₩ 54,000 추가
2 Lsp1109 I R113M 5 units/μl 400 units ₩ 97,000 추가
3 Lsp1109 I R113L 5 units/μl 1,000 units ₩ 216,000 추가
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Recognition & cleavage sequence


Source: Listeria species RFL1109

 

Reaction conditions

1X EzBuffer III, 37

1X FastCut Buffer, 37

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5~15 min with FastCut Buffer

 

1X EzBuffer III

50 mM Tris-HCl (pH 7.9 @ 25), 100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA in 1 hour at 37 in a total reaction volume of 50 µl.

 

Storage

10 mM Tris-HCl (pH 7.4), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

 

Dilution buffer: EzDiluent A

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

Lsp1109 I can be inactivated at 65 for 20 min.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 25%

EzBuffer II: 75%

EzBuffer III: 100%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

Lsp1109 I may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.  Reaction condition of excess enzyme may result in star activity.

● Material Safety Data Sheet

- Enzynomics_MSDS_R113_Lsp1109 I

 

● Quality control

Overdigestion assay

No detectable change in the specific fragmentation pattern is observed after a 5-fold overdigestion with Lsp1109 I.

 

Endonuclease assay

Less than 5% of 1-μg of ΦX174 RFI is converted to RFII when the DNA is incubated with 50 U of enzyme at 37 for 4hr in 50-μl reaction.

 

Ligation and recutting

More than 95% of DNA fragments (1 μg) digested with 2-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, > 50% can be re-cut.

 

Extreme pure (EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

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