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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

Mbo I

No Product Name Cat.# Conc Size Price(₩) Add
1 Mbo I R050S 4 units/µl 500 units ₩ 90,000 추가
2 Mbo I R050M 4 units/µl 1,000 units ₩ 162,000 추가
3 Mbo I R050L 4 units/µl 2,500 units ₩ 360,000 추가
4 Mbo I R050H 20 units/µl 2,500 units ₩ 360,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Recognition & cleavage sequence


Source: Moraxella bovis

 

Reaction conditions

1X EzBuffer III, 37

1X FastCut Buffer, 37

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer III

50 mM Tris-HCl (pH 7.9 @ 25), 100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ at 37 for 1 hr in 50-μl reaction mixtures.

 

Storage

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Dilution buffer: EzDiluent A

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

Mbo I can be inactivated at 65 for 20 min.

 

Methylation sensitivity

dam methylation: sensitive

dcm methylation: Not sensitive

CpG methylation: Conditionally sensitive

 

Prolonged incubation

A minimum amount of enzyme required to digest 1-μg substrate DNA for 16 hr; 0.5 U.

 

Relative activity in EzBuffers

EzBuffer I: 75%

EzBuffer II: 100%

EzBuffer III: 100%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

It is an isoschizomer of Dpn II and Sau3A I. It leaves a 5' GATC extension, which can be efficiently ligated into DNA cleaved by BamH I, Bcl I, Bgl ll, BstY l, Dpn II, or Sau3A I. Cleavage is blocked by dam methylation. Cleavage of mammalian genomic DNA is impaired by CpG methylation partially overlapping its recognition sequence.

 

● Material Safety Data Sheet

- Enzynomics_MSDS_R050_Mbo I

 

● Quality control

Overdigestion assay

DNA digested for 16 hr in 50-μl reaction with 100 U of enzyme resulted in same the DNA band patterns as those obtained with 1 U of enzyme for 1 hr.

 

Endonuclease assay

Less than 5% of 1-μg of ΦX174 RFI is converted to RFII when the DNA is incubated with 50 U of enzyme at 37 for 4hr in 50-μl reaction. 

 

Ligation and recutting

More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, > 95% can be re-cut. 

 

Extreme pure (EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

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