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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

Nt.BstNB I

No Product Name Cat.# Conc Size Price(₩) Add
1 Nt.BstNB I R097S 10 units/μl 1,000 units ₩ 73,000 추가
2 Nt.BstNB I R097M 10 units/μl 2,000 units ₩ 131,400 추가
3 Nt.BstNB I R097L 10 units/μl 5,000 units ₩ 292,000 추가
4 Nt.BstNB I R097H 50 units/μl 5,000 units ₩ 292,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Recognition & cleavage sequence


Source: Bacillus stereothermophilus 33M

 

Reaction conditions

1X EzBuffer III, 55

1X FastCut Buffer, 55

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer III

50 mM Tris-HCl (pH 7.9 @ 25), 100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ at 55 for 1 hr in 50-μl reaction mixtures.

 

Storage

10 mM Tris-HCl (pH 7.5 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Dilution buffer: EzDiluent A

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

Nt.BstNB I can be inactivated at 80 for 20 min.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Prolonged incubation

A minimum amount of enzyme required to digest 1-μg substrate DNA for 16 hr; 0.5U.

 

Relative activity in EzBuffers

EzBuffer I: 0%

EzBuffer II: 10%

EzBuffer III: 100%

EzBuffer IV: 0%

FastCut Buffer: 100%

● Material Safety Data Sheet

- Enzynomics_MSDS_R097_Nt.BstNB I

 

● Quality control

Overdigestion assay

DNA digested for 16 hr in 50-μl reaction with 100 U of enzyme resulted in same the DNA band patterns as those obtained with 1 U of enzyme for 1 hr.

 

Ligation and recutting

More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, > 95% can be re-cut. 

 

Extreme pure (EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

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