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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

Cfr9 I (Xma I)

No Product Name Cat.# Conc Size Price(₩) Add
1 Cfr9 I R081S 10 units/µl 300 units ₩ 78,000 추가
2 Cfr9 I R081M 10 units/µl 600 units ₩ 140,400 추가
3 Cfr9 I R081L 10 units/µl 1,500 units ₩ 312,000 추가
4 Cfr9 I R081H 50 units/µl 1,500 units ₩ 312,000 추가
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Recognition & cleavage sequence


Source: Citrobacter freundii RFL9


IsoschizomerPspA I,TspM I, Xma I, XmaC I

 

Reaction conditions

1X EzBuffer III, 37

 

1X EzBuffer III

50 mM Tris-HCl (pH 7.9 @ 25), 100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ at 37 for 1 hr in 50-μl reaction mixtures.

 

Storage

10 mM Tris-HCl (pH 7.5 @ 25), 250 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Dilution buffer: EzDiluent A

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

Cfr9 I can be inactivated at 65 for 20 min.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: sensitive

 

Relative activity in EzBuffers

EzBuffer I: Not recommended

EzBuffer II: Not recommended

EzBuffer III: 100%

EzBuffer IV: Not recommended

FastCut Buffer: Not recommended

EzBuffer I, II and IV are not recommended due to star activity

 

Note

It is an isoschizomer of Xma . Cleavage of mammalian genomic DNA is blocked by CpG methylation. Reaction condition of low salt, excess enzyme, excess glycerol (>5%) or high pH (>8.0) may result in star activity. To avoid star activity, do not use Cfr9 I in EZbuffer I, II, or IV.

● Material Safety Data Sheet

- Enzynomics_MSDS_R081_Cfr9 I

 

● Quality control

Overdigestion assay

DNA digested for 16 hr in 50-μl reaction with 10 U of enzyme resulted in same the DNA band patterns as those obtained with 1 U of enzyme for 1 hr.

 

Ligation and recutting

More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, > 95% can be re-cut. 

 

Extreme pure (EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

 

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