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Restriction Endonuclease

Home > PRODUCTS > Restriction Endonuclease

BstY I (Psu I)

No Product Name Cat.# Conc Size Price(₩) Add
1 BstY I R078S 10 units/µl 2,000 units ₩ 67,000 추가
2 BstY I R078M 10 units/µl 4,000 units ₩ 120,600 추가
3 BstY I R078L 10 units/µl 10,000 units ₩ 268,000 추가
4 BstY I R078H 50 units/µl 10,000 units ₩ 268,000 추가
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Recognition & cleavage sequence


Source: Bacillus stearothermophilus Y406

 

IsoschizomerBstX2 I, Mfl I, Psu I, Xho II


Reaction conditions

1X EzBuffer II, 60

1X FastCut Buffer, 60

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer II

10 mM Tris-HCl (pH 7.9 @ 25), 50 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ at 60 for 1 hr in 50-μl reaction mixtures.

 

Storage

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Dilution buffer: EzDiluent A

10 mM Tris-HCl (pH 7.4 @ 25), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

BstY I can be inactivated at 80 for 20 min.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Prolonged incubation

A minimum amount of enzyme required to digest 1-μg substrate DNA for 16 hr; 0.13 U.

 

Relative activity in EzBuffers

EzBuffer I: 50%

EzBuffer II: 100%

EzBuffer III: 75%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

It is an isoschizomer of Xho II. It is not affected by dam, dcm, or mammalian CpG methylation.

● Material Safety Data Sheet

- Enzynomics_MSDS_R078_BstY I

 

● Quality control

Overdigestion assay

DNA digested for 16 hr in 50-μl reaction with 100 U of enzyme resulted in same the DNA band patterns as those obtained with 1 U of enzyme for 1 hr.

 

Endonuclease assay

Less than 5% of 1-μg of ΦX174 RFI is converted to RFII when the DNA is incubated with 50 U of enzyme at 37 for 4hr in 50-μl reaction. 

 

Ligation and recutting

More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, > 95% can be re-cut. 

 

Extreme pure (EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

 

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