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TOPreal™ One-step RT qPCR Kit
(TaqMan Probe)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPreal™ One-step RT qPCR Kit
(TaqMan Probe)
RT430S - 100 reactions ₩ 130,000 추가
2 TOPreal™ One-step RT qPCR Kit
(TaqMan Probe)
RT430M - 500 reactions ₩ 585,000 추가
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Product description

TOPreal™ One-step RT qPCR Kit (TaqMan Probe) is designed for one-step RT qPCR using TaqMan probe for detection. In this kit, a ready-to-use RT qPCR mixture containing 4X concentration of components required for RT qPCR in one tube. For reactions, simply add template RNA and a pair of primers resuspended in water. Also, amplified products are monitored in real time, so there is no need to verify them through electrophoresis after PCR. A reverse transcriptase included in this product is an improved version of M-MLV Reverse Transcriptase. It lacks RNaseH activity, and is highly heat stable. Therefore, this product is most suitable for efficiently synthesizing long cDNA by incubating reactions at elevated (50) temperature since high temperature helps removal of secondary structure present in long RNA transcripts. In addition, HOT Start PCR polymerase. included in this product is a derivative of Taq polymerase which is greatly improved in both fidelity and yield for polymerase chain reaction. Compared to other suppliers worldwide, this product can synthesize cDNA with much (approximately 100 fold) less amounts of total RNA. This become more prominent as cDNA to be synthesized is long.

 

Characteristics

- Single-tube convenience

- Well-suited to analysis of a large number of samples

- Excellent sensitivity

- Not Included ROX reference Dye

 

Applications

- Real-Time PCR

- Virus detection

- Single-cell RT PCR

- Gene-expression analysis

- cDNA library construction

- 3’and 5’ RACE, RT PCR

 

Concentration

- 4X

 

Storage

Stable up to 6 months at -20 (Storage at -20 is recommended).

 

Standard PCR conditions

- RT qPCR mixturea

TOPreal™ One-step RT qPCR kit (TaqMan probe)

5 μl

Template (total RNA, 10 ng ~ 1 μg/μl)

1 μl

Gene specific primers (2~20 pmol/μl)

1 μl

Distilled water

up to 20 μl

aAssemble the reaction mixture on ice

 

- RT qPCR cycle

Reverse Transcription

50

30 min

PCR reaction

Initial denaturation

95

10 min

Denaturation

95

5 sec

Annealing & Elongation

60

30 sec

Number of cycles

30~45 times

 

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