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TOPscript™ One-step RT PCR DryMIX (Dye plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPscript™ One-step RT PCR DryMIX (Dye plus)
(20 μl reactions)
RT421 - 96 tubes ₩ 220,000 추가
2 TOPscript™ One-step RT PCR DryMIX (Dye plus)
(20 μl reactions)
RT422 - 480 tubes ₩ 880,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Product description

This product is TOPscript™ One-step RT-PCR DryMIX (Dye plus) that is aliquoted into PCR tube strips. It contains loading dyes for further convenience of use. Thus, the reaction mixtures after PCR cycles are ready for agarose gelelectrophoresis. TOPscript™ One-step RT PCR DryMIX (Dye plus) is a ready-to-use mixture containing dried components required for RT PCR in one tube. For reactions, simply add template RNA and a pair of primers resuspended in water.

 

Characteristics

- Single-tube convenience

- Well-suited to analysis of a large number of samples.

- Synthesis of long cDNA

- Excellent sensitivity

 

Applications

- Virus detection

- Single-cell RT-PCR

- Gene-expression analysis

- cDNA library construction

- 3’ and 5’ RACE, RT-PCR

 

Components

- Reverse Transcriptase

- Hot-start Taq DNA Polymerase

- dNTP mixture

- One-step RT PCR reaction buffer

- Stabilizer

- Loading dye (xylene cyanol)

 

Storage

Stable up to 1 years at -20 (Storage at -20 is recommended).

 

Standard reaction conditions

- a)RT-PCR mixture

TOPscript™ One-step RT PCR DryMIX(Dye plus)

1 tube

Template (total RNA, 10 ng ~ 1 μg/μl)

X μl

Gene specific primers (2~20 pmol/μl)

1 μl

Sterile water

up to 20 μl

aAssemble the reaction mixture on ice

 

- RT-PCR cycle

Reverse Transcription

50~55

30 min

PCR reaction

Initial denaturation

95

10 min

Denaturation

95

5 sec

b)Annealing

55~65

30~60 sec

Annealing & Elongation

60

30 sec

Number of cycles

30~45 times

When cycles are over, keep the reaction mixture at 4; may add 10 mM EDTA until use to prevent DNA degradation.

b)Recommended annealing temperatures is 5 to 10 below the lower Tm of the two primers used.

 


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