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TOPscript™ RT DryMIX (dT18 plus)
No | Product Name | Cat.# | Conc | Size | Price(₩) | Add |
---|---|---|---|---|---|---|
1 | TOPscript™ RT DryMIX (dT18 plus) | RT200 | - | 96 tubes | ₩ 270,000 |
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2 | TOPscript™ RT DryMIX (dT18 plus) |
RT201 | - | 480 tubes | ₩ 1,080,000 |
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Product
description
TOPscript™ RT DryMIX (dT18 plus) is formulated for
maximal stability of TOPscript™ Reverse
Transcriptase that is dried with reaction buffer, dNTP mixture, stabilizer and
oligo dT primer. This product is ready to use upon the addition of template RNA
in distilled water to each tube. TOPscript™
Reverse Transcriptase is genetically engineered version of M-MLV RT which is
highly thermostable thus can synthesize cDNA at elevated temperatures up to 60℃. This property is very useful when RNA
templates are long and have extensive secondary structures. TOPscript™ Reverse Transcriptase is capable of
synthesizing cDNA longer than 20 kb from messenger RNA.
Characteristics
-
Simplicity: The dried pellet in TOPscript™
RT DryMIX is readily soluble upon addition of
template RNA in water.
- Handling:
Well-suited to analysis of a large number of samples.
- Stability: The presence of a unique stabilizer ensures great stability of TOPscript™ Reverse Transcriptase.
- Synthesis
of long cDNA
- Excellent
sensitivity
Applications
- Synthesize
first-strand cDNA,
- Array
labeling
- cDNA
libraries
- 3’ and 5’
RACE, RT-PCR
- Primer
extension
Components
- TOPscript™ Reverse Transcriptase
- TOPscript™ RT buffer
- dNTP
mixture
- RNase
inhibitor
- Stabilizer
- Oligo (dT)18
Storage
buffer
20 mM
Tris-Cl (pH7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01% NP40, 50% glycerol
Standard
reaction conditions
TOPscript™ RT
DryMIX (dT18 plus) |
1 tube |
a)Template RNA |
X μl |
Sterile water (RNase free) |
up
to 20 μl |
a)Prepare
one of the following RNA template.
- Total RNA: 1 ng~5 μg
- Messenger RNA (mRNA): 1 ng~250 ng
- Specific RNA: 0.01 pg~0.5 μg
→Dissolve the dried pellet by vortexing
and briefly spin down.
→An additional annealing step is
recommended:
∙
Incubate at 42℃ for 5 min.
→Incubate at 42℃~60℃ for 60 min.
→Incubate at 95℃ for 5 min to inactivate the reaction.
Note
TOPscript™ Reverse Transcriptase performs optionally over
the full range of 42℃~60℃. Typically, 50℃
is a good starting point. For RNAs containing secondary structure and other
challenging targets, a synthesis temperature of 60℃ may be used without loss of performance.