Product description

2X TOPsimple™ DyeMIX-Tenuto is similarly formulated to the 2X TOPsimple™ PreMIX-Tenuto except that it contains loading dyes for further convenience of use. Thus, the reaction mixtures after PCR cycles are ready for agarose gel electrophoresis. This product includes nTaq-Tenuto (Cat. P225, P250), and is optimized to amplify DNA longer than 10 kb, which is difficult with common Taq polymearases. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products.

Characteristics

- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)

- A-tail formation at 3’ ends of amplified duplex DNA

Applications

- Amplification of long DNA fragments (>5-15 kb)

- Amplification of high-complexity template DNA such as cDNA and genomic DNA

- Primer extension

- Colony PCR

Components (2X)

- nTaq-Tenuto: 0.2 unit/μl

- nTaq-Tenuto buffer (containing 4 mM MgCl₂)

- dNTP mixture: 0.4 mM each

- Stabilizer

- Dyes: Xylene cyanol and Orange G

Migration of dyes in agarose gel

In an ordinary agarose gel, xylene cyanol co-migrate with 4-kb DNA fragments, and Orange G with 50-bp DNA fragments.

Storage

Stable up to 18 months at -20℃ or 3 months at 4℃ (Storage at -20℃ is recommended).

Standard reaction conditions

- PCR mixture^a

^aAssmeble the reaction mixture on ice

^bPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

- PCR cycles

When cycles are over, keep the reaction at 4℃; may add 10 mM EDTA until use to prevent DNA degradation

^aRecommend annealing temperature is 5 to 10℃ below the lower Tm of the two primers used.

●  Enzynomics_P510_2X TOPsimple™ DyeMIX–Tenuto_manual

● Material Safety Data Sheet

- Enzynomics_MSDS_P510_2X TOPsimple™ DyeMIX–Tenuto