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TOPsimple™ DryMIX-HOT

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPsimple™ DryMIX-HOT (20 μl reactions) P581H - 96 tubes ₩ 65,000 추가
2 TOPsimple™ DryMIX-HOT (20 μl reactions) P582H - 960 tubes ₩ 520,000 추가
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Product description

This product is formulated for the maximal stability of nTaq-HOT that has been dried together with reaction buffer, dNTP mixture and a stabilizer. This product is ready to use upon the addition of primers and template DNA in distilled water to each tube.

 

Characteristics

- High Specificity: Formation of nonspecific products or primerdimers is minimized.

- High Sensitivity: Successful PCR with minimal amounts of template.

- Simplicity: The dried pellet in TOPsimple™ DryMIX is readily soluble upon addition of primers and 

  template DNA in water. There is no need of a step to solubilize by mixing.

- Convenience: Dry pellets of TOPsimple™ DryMIX are tightly bound to the bottom of the tubes; 

  easy to transport and handle.

- Stability: The presence of a unique stabilizer ensures great stability of PCR DNA polymerase even at room 

  temperature (up to 2 months).

- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)

- A-tail formation at 3’ ends of amplified duplex DNA

 

Applications

- High specific amplification of DNA fragments shorter than 3 kb.

- Amplification of cDNA and genomic DNA.

- Amplification of template DNA with secondary or higher ordered structure that is resistant to PCR amplification

- Primer extension

- Multiplex PCR

- Well-suited for an automated PCR machines, for which PCR reaction mixtures are prepared at the room 

  temperature

- Colony PCR

 

Components

- nTaq-HOT DNA polymerase: 2 units/tube

- nTaq-HOT buffer (containing 2 mM MgCl2)

- dNTP mixture: 0.2 mM each

- Stabilizer

- Dyes: Xylene cyanol and Orange G

 

Migration of dyes in agarose gel

In an ordinary agarose gel, xylene cyanol migrate with 4-kb DNA fragments, and Orange G with 50-bp DNA fragments.

 

Storage

Stable up to 2 years at -20, 6 months at 4 and 2 months at room temperature (Storage at -20 is recommended).

 

Standard reaction conditions

- PCR mixture

2X TOPsimple™ DryMIX-HOT

1 tube

Template DNAa (0.1~500 ng/μl)

1 μl

Primer 1 (5 pmole/μl)

1 μl

Primer 2 (5 pmole/μl)

1 μl

Sterile water

up to 20 μl

aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

 

- PCR cycles

Step

Temperature

Time

Cycle

Initial denaturationa

95

10 min

1

Denaturation

95

30 sec

25~35

Annealingb

55~65

30~60 sec

Elongation

72

1 min/kb

Final elongation

72

5 min

1

When cycles are over, keep the reaction at 4; may add 10 mM EDTA until use to prevent DNA degradation

aAt least 10 min of initial denaturation time is required to fully activate the chemically modified PCR DNA polymerase

bRecommend annealing temperature is 5 to 10 below the lower Tm of the two primers used.


● Material Safety Data Sheet

- Enzynomics_MSDS_P581H_TOPsimple™ DryMIX-HOT

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