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TOPsimple™ DryMIX–nTaq

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPsimple™ DryMIX–nTaq P581T - 96 tubes ₩ 55,000 추가
2 TOPsimple™ DryMIX–nTaq P582T - 960 tubes ₩ 440,000 추가
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Product Description

This product is formulated for the maximal stability of nTaq that has been dried together with reaction buffer, dNTP mixture and a stabilizer. This product is ready to use upon the addition of primers and template DNA in distilled water to each tube.

 

Characteristics

- Simplicity: The dried pellet in TOPsimple™ DryMIX is readily soluble upon addition of primers and template

  DNA in water. There is no need of a step to solubilize by mixing.

- Convenience: Dry pellets of TOPsimple™ DryMIX are tightly bound to the bottom of the tubes;

  easy to transport and handle.

- Stability: The presence of a unique stabilizer ensures great stability of the PCR DNA polymerase

  even at room temperature (up to 2 months).

- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)

- A-tail formation at 3’ ends of amplified duplex DNA

 

Applications

- Amplification of DNA fragments shorter than 3 kb (Suitable for general PCR analysis)

- Amplification of cDNA and genomic DNA.

- Primer extension

- Colony PCR

 

Components

- nTaq: 2 units/tube

- nTaq buffer (containing 1.5 mM MgCl2)

- dNTP mixture: 0.2 mM each

- Stabilizer

- Dyes: Xylene cyanol and Orange G

 

Migration of dyes in agarose gel

In an ordinary agarose gel, xylene cyanol co-migrate with 4-kb DNA fragments, and Orange G with 50-bp DNA fragments.

 

Storage

Stable up to 2 years at -20, 6 months at 4, or 2 months at room temperature

(Storage at -20 is recommended).

 

Standard reaction conditions

- PCR mixture

TOPsimple™ DryMIX–nTaq

1 tube

Template DNAa (0.1~500 ng/μl)

1 μl

Primer 1 (5 pmole/μl)

1 μl

Primer 2 (5 pmole/μl)

1 μl

Sterile water

up to 20 μl

aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

  

- PCR cycles

Step

Temperature

Time

Cycle

Initial denaturation

95

2 min

1

Denaturation

95

30 sec

25~35

Annealinga

55~65

30~60 sec

Elongation

72

1 min/kb

Final elongation

72

5 min

1

When cycles are over, keep the reaction at 4; may add 10 mM EDTA until use to prevent DNA degradation

aRecommended annealing temperature is 5 to 10 below the lower Tm of the two primers used.



Figure 1. Convenience in transportation and easy to use with TOPsimple DryMIX.

Convenience in transportation; dry pellets containing all PCR components are stably bound to the bottom of tube. Increased solubility; the pellets are readily soluble upon addition of water, making it unnecessary to mix the components prior to PCR reaction.

Figure 2. 2-Dye system for TOPsimple™ DryMIX.

Due to the inclusion of two visible dyes in DryMIX products, tracking of PCR products during electrophoresis is easier and more convenient. Xylene cyanol (XC) and orange G (OG) migrate with 4-kb and 50-bp DNA fragments, respectively



● Material Safety Data Sheet

- Enzynomics_MSDS_P581T_TOPsimple™ DryMIX–nTaq

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