컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

All products

Home > PRODUCTS > All products

EZ™ MEGA T7 Transcription Kit new

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ™ MEGA T7 Transcription Kit EZ039S - 50 rxns ₩ 350,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

Enzynomics EZ™ MEGA T7 Transcription Kit is a product specialized for the synthesis of RNA in high-quantity and high-quality from DNA template containing T7 promoter, and it can simply and efficiently execute the process from synthesis to purification. Per one reaction, ~180 ug of RNA can be produced from 1 ug of linearized template within 1 hr, and total ~7 mg of RNA can be synthesized via using this product, which consists of the materials prepared for 50 reactions. DNA template used in the experiment must contain 19-23 bp of double strand promoter located in its upper part. Once the template is prepared, add RNA polymerase, rNTP, and transcription buffer, and then proceed to the 1 hr reaction at 37°C. Once RNA polymerase binds to the double strand promoter, DNA strands become separated from each other and complementary RNA is synthesized from one template strand in 5`->3` direction. Regardless of whether RNA length is short or long, this product provides successful results in all cases, and it is suitable for various research methods demanding high-quantity RNA synthesis, such as in vitro translation, RNAi, RNase protection asay, RNA splicing, microarray, and in situ hybridization.

 

Kit component

- MEGA T7 Enzyme Mix

- 10X MEGA T7 Reaction Buffer

- rNTP each 100 mM

- Control Template DNA

- Lithium Chloride Precipitation Solution

- DNase I

- 10X RNA Loading Dye

- RNA Sample Buffer

- Ammonium Acetate Stop Solution

- Sterile water (RNase free)

 

Quality control

- Standard reaction produces >100 μg of 1-2 kb RNA transcript per 1 μg of control template in 20 μl for 1 hour

- RNase free

- Endonuclease free

- Exonuclease free



Figure 1. High yield of full-length T7 RNA transcripts

μg of linearized template was used in 20 μl reaction. Standard reactions were incubated at 37℃ in a water bath for hours. Synthesized RNA were purified with LiCl solution and analyzed on 1% agarose gels.


List