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Alw I new

No Product Name Cat.# Conc Size Price(₩) Add
1 Alw I R122S 10 units/μl 500 units ₩ 64,000 추가
2 Alw I R122M 10 units/μl 1,000 units ₩ 115,000 추가
3 Alw I R122L 10 units/μl 2,500 units ₩ 256,000 추가
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Recognition & cleavage sequence

 

Source

Acinetobacter lwoffii

 

Reaction conditions

- 1X EzBuffer , 37

- 1X FastCut Buffer, 37

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer IV

20 mM Tris-acetate (pH 7.9 @ 25), 50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.

 

Storage

10 mM Tris-HCl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

 

Dilution buffer

EzDiluent A

 

Heat Inactivation

No.

 

Methylation sensitivity

dam methylation: Sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 50%

EzBuffer II: 50%

EzBuffer III: 10%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

DNA cleavage is blocked by dam methylation. It produces a 5' extension of one nucleotide, which is more difficult to be ligated than blunt-ends. Reaction condition with excess enzyme, excess glycerol (>5%) or extended digestion may result in star activity.



 


Figure 1. Lambda DNA digested with Alw I



● Material Safety Data Sheet

- Enzynomics_MSDS_R122_Alw I



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