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DNase I, RNase-free new

No Product Name Cat.# Conc Size Price(₩) Add
1 DNase I, RNase-free M059S 10 units/μl 10,000 units ₩ 55,000 추가
2 DNase I, RNase-free M059M 10 units/μl 20,000 units ₩ 99,000 추가
3 DNase I, RNase-free M059L 10 units/μl 50,000 units ₩ 220,000 추가
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Product description

This product is a recombinant form of DNase I from bovine pancreas, expressed in Pichia pastoris. DNase I is a DNA-specific endonuclease that hydrolyses the phosphodiester linkages of double-stranded or single-stranded DNA to a mixture of oligo~ and mononucleotides. The enzyme requires divalent cations for maximal activity. It is a glycoprotein of a molecular weight of ~39 KD. The recombinant enzyme is produced without using any animal cells or other materials derived from animals.

 

Characteristics

- Molecular weight: ~39 Kda

- DNase I is heterogeneously N-glycosylated, so it appears as two bands in gel electrophoresis.

 

Applications

- Remove genomic DNA from RNA preparations prior to RT-PCR 

- Isolate DNA-free RNA after in vitro transcription reactions

- Map DNase-sensitive regions in eukaryotic DNA

- Nick translation

 

Quality control

- Purity: >95% on SDS-PAGE

- RNase-free

- Protease-free

 

Storage buffer

20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, 2 mM MgCl2, 1 mM DTT, 0.1 mg/ml Pefabloc SC,

50% Glycerol, pH 7.6

 

Diluent buffer

25 mM Tris-HCl, 50% Glycerol, pH 7.6

 

Heat inactivation

Inactivated by heating at 75 for 10 min in the presence of EDTA (final 8 mM)

 

Note

DNase I requires divalent cations for maximal activity. The enzyme is inhibited by metal chelating agents like EDTA. DNase I can be inactivated and removed by phenol extraction according to standard protocols.

 

Standard reaction conditions

DNA

1 μg

10X DNase I buffer

2 μl

DNase I

1~2 U

Water, RNase-free

up to 20 μl

→ Incubate at 37 for 10 min.




Figure 1. Genomic DNA digestion by DNase I treatment.

For removal of genomic DNA from RNA samples, a DNase I treatment is recommended. The enzyme is DNA specific without negative effect on the integrity of the remaining RNA.




Figure 2. Cleavage of ss/ds DNA by DNase I.



● Material Safety Data Sheet

- Enzynomics_MSDS_M059_DNase I_E


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