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T3 DNA Ligase

No Product Name Cat.# Conc Size Price(₩) Add
1 T3 DNA Ligase M057S 3,000 unit/μl 150,000 units ₩ 79,000 추가
2 T3 DNA Ligase M057L 3,000 unit/μl 750,000 units ₩ 316,000 추가
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Product description

T3 DNA Ligase is an ATP-dependent dsDNA Ligase derived from Bacteriophage T3. This product catalyzes the phosphodiester linkage between the 3 hydroxyl group and the 5' phosphate group of the dsDNA. It exhibits efficient ligation performance in both cohesive end and blunt end.

 

Applications

- Cloning

- Link adapter, linker to dsDNA

- Circularization of linear DNA

- Nick-sealing of dsDNA

- Site-directed mutagenesis

 

Supplied with

- 2X T3 DNA Ligase Reaction Buffer

 

Storage buffer

10 mM Tris-HCl (pH 7.4), 50 mM KCl, 1 mM DTT, 50% Glycerol, 0.1 mM EDTA

 

Reaction buffer (1X)

66 mM Tris-HCl (pH 7.6),10 mM MgCl2, 1 mM ATP, 1 mM DTT, 7.5% Polyethylene glycol (PEG 6000)

 

Heat inactivation

No.

 

Standard reaction conditions

T3 DNA Ligase

1 μl

2X T3 DNA Ligase Reaction Buffer

10 μl

*Vector DNA (4kb)

0.02 pmol

*Insert DNA (1kb)

0.06 pmol

Distilled water

up to 20 μl

*Vector : Insert = 1 : 3

Incubation at 25 for 15 min




Figure 1. Ligation of λ DNA Hind III fragments by T3 DNA Ligase.

λ DNA Hind III fragments (250 ng) was ligated by incubating at 25℃ for 15 min with increasing amounts of T3 DNA Ligase.




● Material Safety Data Sheet

- Enzynomics_MSDS_M057_T3 DNA Ligase_E

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