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RNase HII new

No Product Name Cat.# Conc Size Price(₩) Add
1 RNase HII M055S 5 units/μl 250 units ₩ 84,000 추가
2 RNase HII M055L 5 units/μl 1,250 units ₩ 336,000 추가
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Product description

Ribonuclease HII (RNase HII) is specifically cleaves RNA only in RNA-DNA duplexes.

 

Characteristics

- Source: E. coli strain

- Reaction temperature: 37

- Heat inactivation: No

 

Applications

- Cleaves RNA only in RNA-DNA duplexes.

 

Supplied with

- 10X RNase HII Reaction Buffer

 

Storage buffer

100 mM NaCl, 20 mM Tris-HCl (pH7.4), 1 mM DTT, 1 mM EDTA, 50% Glycerol

 

Reaction buffer (1X)

20 mM Tris-HCl (pH8.8), 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 1% Triton X-100



Figure1. Cleavage of RNA/DNA Substrates by RNase HII

5’ Radiolabeled dsDNA imbedded single [rU] in the middle was incubated with various amount RNase HII at 37˚C for 15 min. Reaction products were analyzed on 15% denaturing gel 


● Material Safety Data Sheet

- Enzynomics_MSDS_M055_RNase HII_E

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