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SuperiorScript III Master Mix new

No Product Name Cat.# Conc Size Price(₩) Add
1 SuperiorScript III Master Mix RT300S 5X 50 reactions ₩ 360,000 추가
2 SuperiorScript III Master Mix RT300M 5X 250 reactions ₩ 1,440,000 추가
3 SuperiorScript III Master Mix RT300L 5X 500 reactions ₩ 2,520,000 추가
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Product description

SuperiorScript III Master Mix is ready-to-use a First-Strand cDNA synthesis mixture containing SuperiorScript III RT, RNase Inhibitor, a proprietary helper protein, random primers, MgCl2, and dNTPs. This formulation can be used with trace and very high amounts of input RNA (up to 2.5 μg total RNA in a 20 μL reaction). SuperiorScript III Reverse Transcriptase is genetically engineered version of M-MLV RT which is reduced RNase H activity and increased thermostability, thus this enzyme can synthesize first-strand cDNA at elevated temperatures up to 55 . SuperiorScript III Reverse Transcriptase shows improved cDNA yields and RNA detection sensitivity than the competitor’s enzyme. This enzyme is capable of synthesizing cDNA from 100 bp to 12 kb or more.

 

Applications

- First-Strand cDNA synthesis

- Conventional PCR

- Real-time quantitative RT-PCR (qRT-PCR)

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

- Inhibitor-free

- Satisfactory yield and length of cDNA products

 

Composition

- SuperiorScript III Reverse Transcriptase

- SuperiorScript III RT Buffer

- dNTP mixture

- RNase inhibitor

- Random primers

- Helper protein

 

Standard reaction conditions

SuperiorScript III Master Mix

4 μl

Template RNA

X μl

Sterile water (RNase free)

up to 20 μl

→ incubate at 25°C for 10 min and 42 for 60 min.

→ lncubate at 85 for 5 min to inactivate the reaction.

 

Note

- High quality RNA is needed for accurate quantification in qPCR. RNA should be kept in the

abscence of RNase contamination.

- Template RNA can range up to 2.5 μg in a 20μL cDNA synthesis reaction.

- Amplification Grade DNase I can be used to remove genomic DNA contamination from the total RNA.

- Shorter incubation times and/or higher temperatures may be used (e.g., 50°C for 30 minutes),

but may result in reduced yields of cDNA.

- Longer incubation times may be used for increased yields of cDNA.

- Undiluted synthesized cDNA may be used up to 10% of the qPCR reaction volume

(e.g., for a 20 μL qPCR, use up to 2 μL of undiluted cDNA).

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