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[진행] EZ-Fusion HT Cloning Kit 출시 이벤트 ! ...
[18/07/25]
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[18/07/25]
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[18/07/09]
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[18/07/09]
Mun I 
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | Mun I | R118S | 10 units/μl | 300 units | ￦ 45,000 |
|
| 2 | Mun I | R118M | 10 units/μl | 600 units | ￦ 81,000 |
|
| 3 | Mun I | R118L | 10 units/μl | 1,500 units | ￦ 180,000 |
|
Recognition & cleavage sequence
Source
Mycoplasma unidentified
Reaction conditions
- 1X EzBuffer Ⅱ, 37℃
- 1X FastCut Buffer, 37℃
FastCut Buffer
Enzynomics restriction enzyme can cut substrate DNA in 5-15
min with FastCut Buffer
1X EzBuffer II
10 mM Tris-HCl (pH 7.9 @ 25℃), 50 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA
Unit definition
One unit is defined as the amount of enzyme required to
digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50
µl.
Storage
10 mM Tris-HCl (pH 7.4), 100 mM KCl,
1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol
Dilution buffer
EzDiluent A
Heat Inactivation
Mun I can be inactivated at 65℃ for 20 min.
Methylation sensitivity
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG methylation: Not sensitive
Relative activity in EzBuffers
EzBuffer I: 100%
EzBuffer II: 100%
EzBuffer III: 10%
EzBuffer IV: 100%
FastCut Buffer: 100%
Note
It is an isoschizomer of Mfe I.
● Material Safety Data Sheet
