Recognition & cleavage sequence

Source

Mycoplasma unidentified

Reaction conditions

- 1X EzBuffer Ⅱ, 37℃

- 1X FastCut Buffer, 37℃

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

1X EzBuffer II

10 mM Tris-HCl (pH 7.9 @ 25℃), 50 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA

Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Storage

10 mM Tris-HCl (pH 7.4), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

Dilution buffer

EzDiluent A

Heat Inactivation

Mun I can be inactivated at 65℃ for 20 min.

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

Relative activity in EzBuffers

EzBuffer I: 100%

EzBuffer II: 100%

EzBuffer III: 10%

EzBuffer IV: 100%

FastCut Buffer: 100%

Note

It is an isoschizomer of Mfe I.

●  Enzynomics_R118_Mun I_manual

● Material Safety Data Sheet

- Enzynomics_MSDS_R118_Mun I