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Exonuclease T

No Product Name Cat.# Conc Size Price(₩) Add
1 Exonuclease T M052S 5 unit/μl 250 unit ₩ 82,000 추가
2 Exonuclease T M052L 5 unit/μl 1,250 unit ₩ 328,000 추가
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Description

Exonuclease T is an enzyme that removes single stranded RNA (ssRNA) or DNA nucleotides in the 3 '-> 5' direction.

 

Characteristics

- Source: E. coli strain

- Molecular weight: 27 kDa

- Reaction temperature: 25

- Heat inactivation: 65 for 20 min

 

Applications

- Blunt end formation of RNA or DNA with 3 'end

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- No 5'→3' ss and ds Exonuclease Activity

 

10X Reaction buffer

500mM Potassium Acetate, 200 mM Tris-acetate, 100 mM Magnesium Acetate, 10 mM DTT

 

Storage buffer

10 mM Tris-HCl (pH7.4), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol


Figure 1. Cleavage of single-strand DNA by Exonuclease T
5′-end labeled DNA substrates were incubated with various amount of Exonuclease T at 25˚C for 30 min. Reaction products were analyzed on 15% denaturing PAGE.

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