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Acu I

No Product Name Cat.# Conc Size Price(₩) Add
1 Acu I R117S 5 units/μl 300 units ₩ 74,000 추가
2 Acu I R117M 5 units/μl 600 units ₩ 133,000 추가
3 Acu I R117L 5 units/μl 1,500 units ₩ 296,000 추가
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Recognition & cleavage sequence

 

Source: Acinetobacter calcoaceticus

 

Reaction conditions

- 1X EzBuffer , 40 uM S-adenosylmethionine (SAM), 37

- 1X FastCut Buffer, 40 uM S-adenosylmethionine (SAM), 37

 

FastCut Buffer

Enzynomics restriction enzyme can cut substrate DNA in 5-15 min with FastCut Buffer

 

1X EzBuffer IV

20 mM Tris-acetate (pH 7.9 @ 25), 50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

 

Storage

10 mM Tris-HCl (pH 7.4), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

 

Dilution buffer: EzDiluent B

10 mM Tris-HCl (pH 7.4 @25 ), 300 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/μl BSA

50% glycerol

 

Heat Inactivation

Acu I can be inactivated at 65 for 20 min.

 

Methylation sensitivity

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 50%

EzBuffer II: 50%

EzBuffer III: 75%

EzBuffer IV: 100%

FastCut Buffer: 100%

 

Note

Acu I requires S-adenosylmethionine (SAM) for optimal activity. SAM (in 0.005 M sulfuric acid and 10% Ethanol) stored at -20 is stable for at least 6 months. Reaction condition with excess enzyme, excess glycerol (>5%) or longterm incubation may result in star activity.

● Material Safety Data Sheet

- Enzynomics_MSDS_R117_Acu I

 

● Quality control

Overdigestion assay

No detectable change in the specific fragmentation pattern is observed after a 5-fold overdigestion with Acu I.

 

Ligation and recutting

More than 50% of DNA fragments (1 μg) digested with 10-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, ~75% can be re-cut.

 

Extreme pure(EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

 

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