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nTaq-Tenuto (Uracil+) new

No Product Name Cat.# Conc Size Price(₩) Add
1 nTaq-Tenuto (Uracil+) P22501 5 units/µl 250 units ₩ 84,000 추가
2 nTaq-Tenuto (Uracil+) P25001 5 units/µl 500 units ₩ 138,000 추가
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Product description

nTaq-Tenuto (Uracil+) is nTaq (Cat. P025, P050) supplemented with 3'5 proofreading activity and a PCR enhancing factor for improved efficiency and fidelity. nTaq-Tenuto can be used to amplify DNA longer than 10 kb, which is difficult with common Taq polymerases alone. Thus, this product is improved in both the fidelity (> 2 fold) of PCR products and the amplification efficiency of longer PCR products. The uracil binding pocket of proof-reading DNA polymerase in nTaq-Tenuto (Uracil+) is inactivated to enable the amplification of uracil-containing DNA. The enzyme shows the high yield and fidelity, making it advantageous for bisulfite DNA conversion or uracil containing NGS library generation.

 

Characteristics

- Molecular weight: 94 kDa

- Error rate: 3.0 X 10-6

- Thermal stability: Half life of 40 min at 95

- A-tail formation at 3 ends of amplified DNA products.

 

Applications

- Amplification of long DNA fragments

- Amplification of high-complexity template DNA

- Primer extension

- Colony PCR

- Labeling of DNA fragments with radioactive-isotopes

- Nucleotide sequencing

- Methylation analysis amplification of bisulfite-converted DNA for sequencing

- Prevention of false-positive results due to carryover amplicon contamination

- Amplification of damaged DNA samples

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase free

- Inhibitor-free


Supplied with

- 10X nTaq-Tenuto Buffer (Mg2+ plus)

- dNTP Mixture (2 mM each)

- Sterile water



● Material Safety Data Sheet

- Enzynomics_MSDS_P22501_nTaq-Tenuto (Uracil+)

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