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T7 Endonuclease I

No Product Name Cat.# Conc Size Price(₩) Add
1 T7 Endonuclease I M048S 10 unit/μl 250 units ₩ 80,000 추가
2 T7 Endonuclease I M048L 10 unit/μl 1,250 units ₩ 320,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Product description

T7 Endonuclease I (T7 Endo I), the resolvase from T7 phage of Escherichia coli, belongs to the endonuclease family. This product is an engineered form of T7 Endo I. It recognizes and cleaves mismatched DNA, cruciform DNA, heteroduplex DNA, four-way junctions, as well as nicked double-stranded DNA. The site of cleavage is at the first, second or third phosphodiester bond that is 5´ to the mismatch.

 

Characteristics

- The resolvase from T7 phage of bacterium Escherichia coli

 

Applications

- Mutation detection assay

- Detection and cleavage of heteroduplex or nicked DNA

- Linear DNA cleavage for shotgun cloning

 

Quality control

- Endonuclease-free

- Exonuclease-free


Heat inactivation

No


Supplied with

- 10X EzBuffer II

- Sterile water

 



Figure 1. Assaying the formation of digestion product by T7 Endonuclease I

Digestion mixtures containing heterozygous mutant and T7 Endonuclease I were incubated at 37 for 15 min. Reaction products were analyzed on 1.5% agarose gel and stained with EtBr.

● Material Safety Data Sheet

Enzynomics_MSDS_M048_T7 Endonuclease I_E

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