Product description

2X RbTaq™ PreMIX-HOT is a ready-to-use PCR mixture containing 2X concentration of hot start DNA polymerase, reaction buffer, and dNTP. This product includes RbTaq™ HOT (Cat. P725RB), and is optimized to increase the specificity and efficiency of target DNA amplification

Characteristics

- A-tail formation at 3’ ends of amplified duplex DNA

Applications

- High specific amplification of DNA fragments shorter than 3 kb.

- Amplification of cDNA and genomic DNA.

- Amplification of template DNA with secondary or higher ordered structure that is resistant to PCR amplification

- Primer extension

- Well-suited for an automated PCR machines, for which PCR reaction mixtures are prepared at the room 

  temperature

- Colony PCR

- Labeling of DNA fragments with radioactive-isotopes

- Nucleotide sequencing

Components(2X)

- RbTaq™ HOT DNA Polymerase: 0.2 unit/μl

- RbTaq™ HOT Buffer (containing 4 mM Mg²⁺)

- dNTP mixture: 0.4 mM each

- Stabilizer

Storage

Stable up to 18 months at -20℃ or 3 months at 4℃ (Storage at -20℃ is recommended).

Standard reaction conditions

- PCR mixture

^aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

- PCR cycles

When cycles are over, keep the reaction at 4℃; may add 10 mM EDTA until use to prevent DNA degradation

^a30 sec at 95℃ for cDNA and 3 min at 95℃ for genomic DNA is recommended for enzyme activation.

^bRecommend annealing temperature is 5 to 10℃ below the lower Tm of the two primers used.

●  Enzynomics_P610HRB_2X RbTaq™ PreMIX-HOT_manual

● Material Safety Data Sheet

- Enzynomics_MSDS_P610HRB_2X RbTaq™ PreMIX-HOT