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EZ™ Thermo Sequenase

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1 EZ™ Thermo Sequenase TS100 8 units/μl 1,000 units ₩ 216,000 추가
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Product Description

EZ™-Thermo Sequenase is a Taq DNA polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides.

 

Source

An E. coli strain that carries the modified Taq DNA polymerase gene, a genetically engineered form of the native DNA polymerase from Thermus aquaticus.

 

Characteristics

- Molecular weight: 65 KDa

 

Applications

- DNA Sequencing

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease free

- Exonuclease free

 

Unit definition

One Units is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble materials in 30 min at 74 in 50 μl reaction mixture.

 

Storage buffer

20mM Tris-HCl (pH 8.5), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween 20, 50% glycerol.

 

10X EZ™-Thermo Sequenase Buffer

260 mM Tris-HCl (pH 9.5), 65 mM MgCl2

● Material Safety Data Sheet

- Enzynomics_MSDS_TS100_EZ™ Thermo Sequenase

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