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RbTaq™ qPCR 2X PreMIX
(TaqMan Probe for multiplex) new

No Product Name Cat.# Conc Size Price(₩) Add
1 RbTaq™ qPCR 2X PreMIX
(TaqMan Probe for multiplex)
RT650S 2X 200 reactions ₩ 180,000 추가
2 RbTaq™ qPCR 2X PreMIX
(TaqMan Probe for multiplex)
RT650M 2X 500 reactions ₩ 360,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

RbTaq™ qPCR 2X PreMIX (TaqMan Probe for multiplex) is a preassembled liquid mixture that contains hot-start Taq, optimal reaction buffer, dNTP and stabilizing agents except primers, probes, and template. RbTaq™ qPCR 2X PreMIX (TaqMan Probe for multiplex) is a repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, Taq DNA polymerse is combined with a repebody that specifically binds to the enzyme and inactivates until the first denaturation step. An optimized multiplex PCR master mix designed specifically for Real-time PCR, which makes it possible to detect multiple targets at the same time.

 

Characteristics

- High-speed: Repebody mediated hot start Taq allows rapid and accurate qPCR

- Multiplexing

- Ease of Use: Premix contains everything needed except for primers, template and probe.

- High specificity: Enhanced specificity by the use of HOT Start PCR polymerase.

- Stability: Enhanced stability allows one year storage at -20

- Included 100 nM ROX reference Dye

 

Applications

- Pathogen Identification

- High Throughput SNP Genotyping

- Gene Deletion Analysis

- Template Quantitation

- Linkage Analysis

 

Standard reaction conditions

- PCR mixture

RbTaq™ qPCR 2X PreMIX (TaqMan Probe for multiplex)

10 μl

Template DNA

1 μl

Probe (2~4 pmol/μl)

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Initial denaturationa

95

3 min

Denaturation

95

≤ 10 sec

Annealing & Elongation

60

≤ 60 sec

Number of cycles

35~40 times

a 30 sec at 95 for cDNA and 3 min at 95 for genomic DNA is recommended for enzyme activation.

 

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