컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

All products

Home > PRODUCTS > All products

EZ™ dA-Tailing Module

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ™ dA-Tailing Module EZ032S - 20 reactions ₩ 118,000 추가
2 EZ™ dA-Tailing Module EZ032L - 100 reactions ₩ 472,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

EZ™ dA-Tailing Module is used to add a non-templated dAMP on the 3’ end of a blunt DNA fragment. Incorporated 3’-dA prevents concatamer formation during subsequent ligation of adaptors or cloning vectors with complementary dT overhangs. This kit has been optimized to prepare compatible overhangs for next generation sequencing.

 

 

Characteristics

Master mix type for dA-tailing in DNA sample preparation workflows.

 

Applications

- DNA sample preparation

- dA-Tailing of 1-5 μg fragmented DNA

 

Standard reaction conditions

End repaired DNA(100-1000 bp)

1~5 μg

Klenow DNA polymerase, exo-

3 μl

10X dA-Tailing Reaction Buffer

5 μl

Sterile water

up to 50 μl

Incubate in a thermal cycler for 30 minutes at 37°C



 

   

Figure 1. dA-tailing of repaired blunt end DNA

Reaction mixtures with 100 pmol of repaired blunt end DNA substrates were incubated with EZTM dA-Tailing Module for 30 min at 37. For the assessment of dA-Tailing reaction efficiency, self ligation reaction was performed with 20 pmol of dA-tailed DNA. Reaction products were analyzed on 10% native PAGE.

 

List