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EZ™ dA-Tailing Module
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | EZ™ dA-Tailing Module | EZ032S | - | 20 reactions | ￦ 118,000 |
|
| 2 | EZ™ dA-Tailing Module | EZ032L | - | 100 reactions | ￦ 472,000 |
|
Product
description
EZ™
dA-Tailing Module is used to add a non-templated dAMP on the 3’ end of a blunt
DNA fragment. Incorporated 3’-dA prevents concatamer formation during
subsequent ligation of adaptors or cloning vectors with complementary dT
overhangs. This kit has been optimized to prepare compatible overhangs for next
generation sequencing.
Characteristics
Master
mix type for dA-tailing in DNA sample preparation workflows.
Applications
-
DNA sample preparation
-
dA-Tailing of 1-5 μg fragmented DNA
Standard
reaction conditions
|
End
repaired DNA(100-1000 bp) |
1~5 μg |
|
Klenow
DNA polymerase, exo- |
3 μl |
|
10X
dA-Tailing Reaction Buffer |
5 μl |
|
Sterile
water |
up to 50 μl |
→
Incubate in a thermal cycler for 30 minutes at 37°C
Figure
1. dA-tailing of repaired blunt end DNA
Reaction
mixtures with 100 pmol of repaired blunt end DNA substrates were incubated with
EZTM dA-Tailing Module for 30 min at 37℃. For the assessment of dA-Tailing
reaction efficiency, self ligation reaction was performed with 20 pmol of
dA-tailed DNA. Reaction products were analyzed on 10% native PAGE.
● Material Safety Data Sheet
