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EZ™ Adaptor Ligation Module

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ™ Adaptor Ligation Module EZ031S - 20 reactions ₩ 303,000 추가
2 EZ™ Adaptor Ligation Module EZ031L - 100 reactions ₩ 1,212,000 추가
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Product description

EZ™ Adaptor Ligation Module is formulated for quickly ligate DNA adaptors to blunt or dA-Tailed DNA fragments. The product provides efficient performance and room temperature reaction setup in a convenient master mix.



Characteristics

Master mix type ligase for DNA adaptor ligation

 

Applications

- Ligate DNA adaptors to blunt or dA-tailed DNA fragments

- DNA Sample Preparation for NGS

- Ligation of blunt or dA-Tailed DNA and cloning vectors.

 

Standard reaction conditions

End repaired or A-Tailed DNA

Variable

Adaptor DNA

Variable

TOPspeed™ DNA Ligase

5 μl

5X Adaptor ligation Reaction Buffer

10 μl

Sterile water

Up to 50 μl

Incubate in a thermal cycler for 15 minutes at 20°C

 


      

      


Figure 1. The result of adaptor ligation reaction

30-nt dT-tailed dsDNA adaptors were covalently attached to 20-nt dA-tailed dsDNA using EZTM Adaptor Ligation Module. Reaction mixtures were incubated at 25 for 15 min, followed by analyzed on 10% denaturing PAGE.

 

 

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