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T4 RNA Ligase 2, truncated KQ

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 RNA Ligase 2, truncated KQ M046S 200 units/μl 4,000 units ₩ 77,000 추가
2 T4 RNA Ligase 2, truncated KQ M046L 200 units/μl 20,000 units ₩ 308,000 추가
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Product description

T4 RNA Ligase 2, truncated KQ is a double-point mutant of T4 RNA Ligase 2, truncated containing K227Q and R55K mutation. This enzyme ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA. The enzyme does not use ATP for ligation but requires pre-adenylated linkers. Mutation of R55K in T4 Rnl2, truncated K227Q increases the ligation activity of the enzyme to levels similar to T4 Rnl2 truncated


Characteristics

- The double-point mutant form of T4 RNA Ligase 2, truncated from E. coli


Applications

- Ligate pre-adenylated 5´ end of DNA or RNA to the 3´end of RNA


Supply with

- 10X T4 RNA Ligase Reaction Buffer

- PEG8000

- Sterile water


Standard reaction conditions

1X T4 RNA Ligase Reaction Buffer, Incubate at 25°C



Figure 1. Assaying the formation of ligation product by T4 RNA Ligase 2, truncated KQ

Ligation mixtures containing 5’pre-adenylated DNA adapter, 30 mer RNA acceptor and ligase were incubated at 25℃ for 2 hr. Reaction products were analyzed on 15% denaturing PAGE and stained with EtBr.


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