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T4 RNA Ligase 2, truncated

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 RNA Ligase 2, truncated M044S 200 units/μl 4,000 units ₩ 78,000 추가
2 T4 RNA Ligase 2, truncated M044L 200 units/μl 20,000 units ₩ 312,000 추가
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Product description

T4 RNA ligase 2 catalyzes the joining of a 3′-hydroxyl terminus of RNA to a 5′-phosphorylated RNA or DNA. Unlike RNA ligase 1, this enzyme prefers double-stranded RNA or double-stranded RNA/DNA hybrids.


Characteristics

- Double-strand RNA Ligase from an E. coli.

 

Applications

- Ligate nick in double-stranded RNA.

- Ligate 3' OH of RNA to 5' phosphate of DNA in a DNA/RNA hybrid.


Supply with

- 10X T4 RNA Ligase Reaction Buffer

- PEG8000

- Sterile water


Standard reaction conditions

1X T4 RNA Ligase Reaction Buffer, Incubate at 25°C



Figure 1. Assaying the formation of ligation product by T4 RNA Ligase 2, truncated

Ligation mixtures containing 5’pre-adenylated DNA adapter, 30 mer RNA acceptor and ligase were incubated at 25℃ for 2 hr. Reaction products were analyzed on 15% denaturing PAGE and stained with EtBr.


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