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T4 RNA Ligase 2

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 RNA Ligase 2 M043S 10 units/μl 300 units ₩ 85,000 추가
2 T4 RNA Ligase 2 M043L 10 units/μl 1,500 units ₩ 340,000 추가
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Product description

T4 RNA ligase 2 catalyzes the joining of a 3′-hydroxyl terminus of RNA to a 5′-phosphorylated RNA or DNA. Unlike RNA ligase 1, this enzyme prefers double-stranded RNA or double-stranded RNA/DNA hybrids.


Characteristics

- Double-strand RNA Ligase from an E. coli.

 

Applications 

- Ligate nick in double-stranded RNA.

- Ligate 3' OH of RNA to 5' phosphate of DNA in a DNA/RNA hybrid.


Supply with

- 10X T4 RNA Ligase 2 Reaction Buffer,

- Sterile water


Standard reaction conditions

1X T4 RNA Ligase 2 Reaction Buffer, Incubate 30 min at 37°C



Figure 1. Assaying the formation of ligation product by T4 RNA Ligase 2

20 pmol of dsDNA/RNA hybrid substrates were incubated with T4 RNA Ligase 2 for 30 min at 37℃. Reaction products were Analyzed on 15% denaturing PAGE and stained with EtBr.


● Material Safety Data Sheet

- Enzynomics_MSDS_M043_T4 RNA Ligase 2_E

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