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5X Lambda Exonuclease Mix

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1 5X Lambda Exonuclease Mix M041S - 150 reactions ₩ 60,000 추가
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Product description

5X Lambda Exonuclease Mix is ready-to-use solution of Lambda Exonuclease for convinient use. 5 μl of 5X Lambda Exonuclease Mix can completely cleavage 1 μg of DNA in 25 ul reaction volumn within 30 min at 37.

 

Characteristics

- 5X Ready-to-use solution of Lambda Exonuclease.

 

Applications

- Generation of ssDNA sequencing template from PCR products.

- Restriction mapping.

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

 

Standard reaction conditions

PCR Product or DNA(0.1 μg – 1 μg)

20 μl

5X Lambda Exonuclease Mix

5 μl

Total

25 μl

 



Figure 1. Degradation of phosphorylated DNA

Varying amount of restriction digested (pSKMII-Hind III) linear dsDNAs were degraded by both Lambda Exonuclease (5 unit) and Lambda Exonuclease Mix at 37 for 15 min.

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