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5' DNA Adenylation Kit

No Product Name Cat.# Conc Size Price(₩) Add
1 5' DNA Adenylation Kit EZ030S 50 μM 10 rxns ₩ 127,000 추가
2 5' DNA Adenylation Kit EZ030L 50 μM 50 rxns ₩ 508,000 추가
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Product description

5’ DNA Adenylation Kit is used for the enzymatic synthesis of 5’ adenylated ssDNA linkers. This kit is optimized to generate the adenylated DNA with or without 3-terminator.

 

Characteristics

Mth RNA Ligase is purified from an E. coli carrying a plasmid encoding thermostable RNA ligase from Methanobacterium thermoautotrophicum.

 

Applications

Enzymatic 5’ adenylation of single-stranded DNA linkers for next generation sequencing

 

Supplied with

- Mth RNA Ligase

- 10X 5’ DNA Adenylation Reaction Buffer

- 1 mM ATP

- Sterile water



Figure 1.  Adenylation activity of Mth RNA Ligase

Adenylation mixtures containing 3’amino blocked DNA and ligase with 0.1 mM ATP were incubated at 65℃ for 1 hr. Reaction products were analyzed on 15% denaturing PAGE and stained with EtBr.

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