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EZ™ USE Enzyme

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ™ USE Enzyme M040S 1 unit/μl 50 units ₩ 82,000 추가
2 EZ™ USE Enzyme M040L 1 unit/μl 250 units ₩ 328,000 추가
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Product description

Enzynomics Uracil-Specific Excision (USE) enzyme consists of Uracil DNA glycosylase (UDG) and DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, creating the abasic (apyrimidinic) site. The lyase activity of Endonuclease VIII then breaks the phosphodiester back bone at the 3´ and 5´ sides of the abasic site.


Characteristics

Enzyme mix containing Uracil DNA glycosylase (UDG) and DNA glycosylase-lyase Endonuclease VIII.


Applications

- Gene cloning

- Next Generation Sequencing



Figure 1. Cleavage of DNA containing an uracil base by USE enzyme

20 pmol of 5’ end-labeled DNA substrates  with uracil base were Incubated with USE enzyme for 15 min at 37℃. Reaction products were analyzed on 15% denaturing PAGE.


"Shipping only to Asia."

● Material Safety Data Sheet

- Enzynomics_MSDS_M040_EZ™ USE Enzyme_E

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