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RNase Inhibitor, Murine

No Product Name Cat.# Conc Size Price(₩) Add
1 RNase Inhibitor, Murine M035S 40 units/μl 3,000 units ₩ 76,000 추가
2 RNase Inhibitor, Murine M035L 40 units/μl 15,000 units ₩ 304,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Product description

RNase Inhibitor, Murine is expressed and purified from E. coli as a recombinant. This product strongly inhibits major nonspecific ribonucleases that include RNase A, B and C. However, it does not inhibit sequence-specific ribonuclease or ribonuclease activities associated with DNA or RNA polymerases; these include RNase T1, S1 nuclease and RNase from Aspergillus species. Mouse RNase Inhibitor has significantly improved resistance to oxidation compared to the human RNase inhibitors, and is stable at low DTT concentrations.


Characteristics

- Isolated from a recombinant source

- Improved resistance to oxidation, compared to human RNase inhibitor


Applications

- In vitro transcription 

- cDNA synthesis

- RT qPCR

- In vitro translation

- Separation and identification of specific ribonuclease activities

- Studies of tumor suppression


Quality control

- Purity : >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free


Unit definition

One unit is defined as the amount of mouse RNase Inhibitor required to inhibit the activity of 5 ng of 

RNase A by 50%


Component

mouse RNase Inhibitor

Sterile water


Storage buffer

20 mM HEPES-KOH(pH 7.6), 50 mM KCl, 8 mM DTT, 50% glycerol


Cautions

For efficient protection of RNA in the reaction, add l units fo mouse RNase Inhibitor to 1 μl of reaction mixture.



Figure 1. Reaction mixtures (20 μl) containing 5 µg of RNA were incubated with RNase A in the absence or presence of various amounts of RNase Inhibitor.

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