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PBCV-1 Ligase

No Product Name Cat.# Conc Size Price(₩) Add
1 PBCV-1 Ligase M031S 25 units/μl 1,250 units ₩ 102,000 추가
2 PBCV-1 Ligase M031L 25 units/μl 6,250 units ₩ 408,000 추가
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Product description

PBCV-1 DNA Ligase, the Chlorella virus DNA Ligase is known and mediates the ligation of two nicked single-stranded DNAs annealed to a bridging template RNA strand efficiently. Specifically, it can ligate DNAs consisting of a 5′-phosphate-terminated and a 3′-hydroxyl-terminated strand. This activity can be applied to SNP or RASL-seq.


Characteristics

- Isolated from a recombinant source

- Reaction temperature: 37°C

- Heat inactivation: 65°C for 20 min


Applications

- Ligation of ssDNA splinted by complementary RNA sequences

- Detection of RNA using ligation of DNA probes

- SNP or splice variant detection

- RASL-seq


Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free


Unit definition

One unit is defined as the amount of enzyme needed to ligate (to 50% completion) 2 picomoles of a tripartite FAM-labeled DNA:RNA hybrid substrate in a 20 μl reaction at 25°C in 15 minutes in 1X Reaction Buffer.


Storage buffer

10 mM Tris-HCl (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 20 mM DTT, 50% glycerol.


Reaction buffer (10X)

500 mM Tris-HCl, 100 mM MgCl2, 10 mM ATP, 7.5% PEG-8000, pH 7.4


Figure 1. 20 pmol of 5’ end-labeled ssDNA/RNA substrates were incubated with PBCV-1 Ligase for 15 min at 37℃. Reaction products were Analyzed on 15% denaturing PAGE.



● Material Safety Data Sheet

- Enzynomics_MSDS_M031_PBCV-1 Ligase_E

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