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Acc III

No Product Name Cat.# Conc Size Price(₩) Add
1 Acc III R059S 10 units/µl 1,000 units ₩ 80,000 추가
2 Acc III R059M 10 units/µl 2,000 units ₩ 144,000 추가
3 Acc III R059L 10 units/µl 5,000 units ₩ 320,000 추가
4 Acc III R059H 50 units/µl 5,000 units ₩ 320,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Recognition & cleavage sequence


Source: Acinetobacter calcoaceticus

 

Reaction conditions

1X EzBuffer Acc III 65

 

1X EzBuffer Acc III

10 mM Tris-HCl (pH 8.5 @25), 100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ (dam-)

at 65 for 1 hr in 50-μl reaction mixtures.

 

Storage

10 mM Tris-HCl (pH 7.3 @ 25), 300 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA, 50% glycerol.

 

Dilution buffer: EzDiluent B

10 mM Tris-HCl (pH 7.4 @ 25), 300 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA, 50% glycerol.

 

Heat Inactivation

No.

 

Methylation sensitivity

dam methylation: Conditionally sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Relative activity in EzBuffers

EzBuffer I: 0%

EzBuffer II: 25%

EzBuffer III: 100%

EzBuffer IV: 0%

FastCut Buffer: Not recommended

 

Note

Acc III cleavage is blocked by dam methylation partially overlapping its recognition site.

● Material Safety Data Sheet

Enzynomics_MSDS_R059_Acc III

 

● Quality control

Overdigestion assay

DNA digested for 16 hr in 50-μl reaction with 100 U of enzyme resulted in same the DNA band patterns as those obtained with 1 U of enzyme for 1 hr.

 

Endonuclease assay

Less than 5% of 1-μg of ΦX174 RFI is converted to RFII when the DNA is incubated with 50 U of enzyme at 37 for 4hr in 50-μl reaction.

 

Ligation and recutting

More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16 for 16 hr. Of the ligation products, > 95% can be re-cut.

 

Extreme pure (EP)

No detectable degradation of 32P-end labeled single-stranded and double-stranded (5’-, 3’-overhang and blunt end) oligonucleotides occurred during incubation with 100 U of enzyme for 4 hr.

 

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