-
[18/10/01]
-
[진행] 10월 ! EtBr 대체제 출시 "EZstain DNA Loa...
[18/10/01]
-
[진행] 2018년 9월 출시제품 이벤트(11월 까지)
[18/09/12]
-
[18/07/25]
-
[진행] Premade Buffer 이벤트 2탄! (12월 까지)
[18/07/09]
20X AP-Mix
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | 20X AP-Mix | M033S | 20X | 500 rxns | ￦ 64,000 |
|
This product is a 20X mix version of Antarctic
Phosphatase (Cat. # M026).
Product
description
20X
AP-Mix (Antarctic Phosphatase 20X PreMIX) removes 5´ phosphate from DNA, RNA,
rNTPs and dNTPs. Since phosphatase-treated fragments lack the 5´phosphoryl
termini required by ligases, they cannot self-ligate. This property can be used
to decrease the vector background in cloning strategies.
20X
AP-Mix is ready-to-use solution of Antartic Phosphatase for convinient use. 1
ul of 20X AP-Mix can completely dephosphorylate 1 ug of linearized plasmid DNA
in 20 ul reaction volumn within 1 hr at 37℃.
Characteristics
-
Remove 5´ phosphate from DNA, RNA, rNTPs and dNTPs.
Applications
-
Prevents recircularization of cloning vectors
-
Preparation of templates for 5´ end labeling
-
Removal of dNTPs and pyrophosphate from PCR reactions
-
Dephosphorylation of proteins
Cautions
Avoid
continuous freez and melt cycles
Standard
conditions
|
Linearized plasmid DNA (~2 ug) |
~19 μl |
|
20X AP-Mix |
1 μl |
|
Sterile water |
Up to 20 μl |
→Incubate
at 37℃
for 1 hr.
→Inactivate at 70℃ for 5 min or purify DNA for subsequent application.
Figure 1. 1 μg of pcDNA3.1 cut with Hind III (5´ protruding ends), were incubated with AP mix for 30 min at 37°C. Dephosphorylation is defined as inhibition of recirculation in a self-ligation reaction and is measured by transformation into E. coli.
● Material Safety Data Sheet
