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T4 Gene 32 Protein

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 Gene 32 Protein M027S 10 mg/ml 100 μg ₩ 80,000 추가
2 T4 Gene 32 Protein M027L 10 mg/ml 500 μg ₩ 320,000 추가
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Product description

T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein which is required for T4 DNA replication, recombination, and repair. It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication. T4 Gene 32 Protein has been widely used in studies of DNA-protein interactions and for marking regions of single-stranded DNA in cytological preparations viewed by electron microscopy. Recently, it has been shown to improve restriction enzyme digestion, improve the yield and efficiency of reverse transcription (RT) reactions during RT-PCR, enhance T4 DNA polymerase activity, as well as increase the yield of PCR products.

 

Characteristics

- Isolated from a recombinant source

- Reaction temperature: 37

- Heat inactivation: 65 for 20 min

- Molecular Weight: 33.5 KDa

 

Applications

- Increase yield and specificity of PCR products.

- Increases yield and processivity of reverse transcription.

- Stabilization of single-stranded regions of DNA or RNA.

- Improves restriction enzyme digestion.

- Enhance T4 DNA polymerase activity.

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

 

Component

- T4 Gene 32 Protein

- 10X T4 Gene 32 Protein Buffer

- Sterile water

 

Storage buffer

10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 

 

Reaction buffer (10X)

200 mM Tris-acetate, 100 mM Magnesium acetate, 500 mM potassium acetate, 10 mM DTT, pH 7.9

 

Standard reaction conditions

1X T4 Gene 32 Protein Buffer, incubate at 37°C.



Figure 1. ssDNA binding test of T4 Gene 32 Protein

DNA bound protein complexes were analyzed on 6% polyacrylamide gels in 0.5X TBE at 4°C.

● Material Safety Data Sheet

- Enzynomics_MSDS_M027_T4 Gene 32 Protein_E

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