컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

All products

Home > PRODUCTS > All products

TOPreal™ qPCR 2X PreMIX
(TaqMan Probe, UDG plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPreal™ qPCR 2X PreMIX
(TaqMan Probe, UDG plus)
RT600US 2X 200 rxns ₩ 120,000 추가
2 TOPreal™ qPCR 2X PreMIX
(TaqMan Probe, UDG plus)
RT600UM 2X 500 rxns ₩ 240,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

TOPreal™ qPCR 2X PreMIX (TaqMan Probe, UDG plus) provides accurate real-time quantification of DNA and cDNA targets (100~150 bp in length). High specificity and sensitivity in PCR are achieved by the use of the hot-start enzyme together with a specialized PCR buffer. TOPreal™ qPCR 2X PreMIX (TaqMan Probe, UDG plus) is a liquid mixture that contains Taq Polymerase (for hot start PCR), optimal reaction buffer, dNTP, stabilizing agents. Also, UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions.

 

Characteristics

- Carry-over contamination control: contains UDG

- High specificity: Enhanced specificity by the use of Hot Start PCR polymerase

- Stability: Enhanced stability allows one year storage at -20°C.

- Easy-to-use: PreMIX contains everything needed except for primers, template and probe

- High reproducibility is ensured by rigorous quality control

- Included 100 nM ROX reference Dye

 

Applications

- Real-time quantification of gDNA and cDNA targets

- Gene expression profiling

- Microbial & viral pathogen detection

 

Quality control

- Endonuclease assay

 

Standard reaction conditions

- PCR mixture

TOPreal™ qPCR 2X PreMIX (TaqMan Probe, UDG plus)

10 μl

Template DNA

1 μl

Probe (2~4 pmol/μl)

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Pre-incubation (for UDG)

50

4 min

Initial denaturationa

95

10~15 min

Denaturation

95

10 sec

Annealing & Elongation

60

60 sec

Number of cycles

35~55 times

aAt least 10 min of initial denaturation time is required to fully activate the chemically modified PCR DNA polymerase

 

List