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T3 RNA Polymerase

No Product Name Cat.# Conc Size Price(₩) Add
1 T3 RNA Polymerase RP004S 20 units/μl 2,000 units ₩ 60,000 추가
2 T3 RNA Polymerase RP004L 20 units/μl 10,000 units ₩ 240,000 추가
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Product description

T3 RNA Polymerase is highly specific for its own promoter of a conserved 23 bp sequence. This sequence does not efficiently recognize by SP6 or T7 RNA Polymerases.

Therefore, without cross-talk from nearby SP6 or T7 promoters it will transcribe large amounts of RNA from DNA sequences.

 

Characteristics

- Isolated from an E. coli strain harboring a plasmid that over expresses T3 RNA polymerase

- mRNA generation for in vitro translation systems

 

Applications

- mRNA generation for in vitro translation systems

- Radiolabeled RNA probe preparation

- RNA probe preparation for hybridization

- Genomic sequencing

- RNase protection studied

 

Quality Control

- Endonuclease -free

- Exonuclease-free

- Non-Specific DNase Activity

- RNA Polymerase Specificity:

- RNase Activity

 

Unit Definition

One unit the amount of T3 RNA polymerase required to catalyze the incorporation of 1 nmol of nucleoside triphosphate into acid-insoluble material in 60 min at 37.

 

Storage Conditions

50 mM Tris-HCl (pH8.0), 150 mM NaCl, 5 mM DTT, 0.1 mg/ml BSA, 0.1% Triton X-100,50% glycerol (v/v).

 

Standard reaction conditions
- RNA polymerization conditions

10X T3 RNA Polymerase buffer

μl

T3 RNA polymerase (20 unit/μl)

μl

rNTP mixture (5 mM each)

μl

10X DTT

5 μl

Double-stranded DNA template (1 μg/μl)

1 μl

RNase Inhibitor (40 unit/μl)

1 μl

Sterile water (RNase free)

up to 50 μl

→Incubate at 37 for 60 to 120 min

→Terminate reaction by incubating at 75 for 20 min, or adding 2 μl of 0.5M EDTA

Reagents and materials not provided: rNTP, RNase Inhibitor 

● Material Safety Data Sheet

Enzynomics_MSDS_RP004_T3 RNA Polymerase

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